Composite

Part:BBa_K4940012:Design

Designed by: Kuang Minjie   Group: iGEM23_iZJU-China   (2023-10-11)


Transcription unit of GLF-his


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 506
    Illegal NgoMIV site found at 590
    Illegal NgoMIV site found at 956
    Illegal NgoMIV site found at 989
    Illegal NgoMIV site found at 1025
    Illegal AgeI site found at 545
    Illegal AgeI site found at 701
    Illegal AgeI site found at 713
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The main idea was to have a high expression of the gene and the expression should be controlled safely. In that case, commercial T5 promoter is useful and lac operator represses the gene expression without IPTG induction.

Source

Most of the parts are already in the commercial plasmids, and we select a proper vector to use. The gene sequence was found in NCBI and synthesized by the company with codon optimization.

References